For getting enough eggs for carrying out in vitro fertilization (IVF) procedure, the reproductive organs of the female are stimulated with medications using one of the many IVF stimulus protocols. These protocols help in developing plentiful of follicles and eggs. The medicinal injections are generally given by the woman herself or by her spouse or partner.
The doctor may discuss with you the tentative IVF calendar listing the days on which the stimulus shots are to be given; days of visits to the Clinic and when the egg retrieval and the embryo transfer procedures are to be carried out.
- Eggs grow in the fluid-filled sacs in the ovaries, known as follicles.
- With the help of ultrasound scan, the follicles can be seen and quantified. Every follicle has only one egg.
- The eggs are loosely joined to the follicle walls.
Size of the follicles for carrying out egg retrieval
- As the follicles attain maturity, the egg retrieving procedure is carried out for taking the eggs out. The egg maturity is ascertained by the ultrasound procedure wherein the size of the follicles is measured. Besides, the presence and the quantity of certain hormones in your blood are also measured that indicate the maturity stage of the follicles.
- The follicles that grow to nearly between 15 and 20 mm diameter are generally considered as “mature” follicles.
Anesthesia for egg retrieval
- Domineering anesthesia medicines are given intravenously for keeping the female “out” of the egg retrieval process. The woman, under the influence of sedation, will neither feel any pain nor remember anything.
- Many clinics use other kind of anesthesia, but as per the international standard, New Life India Clinic generally uses just “putting-to-sleep” anesthesia, but the woman involved is able to breathe on her own.
- General anesthesia, conscious sedation or even localized anesthesia may be given for carrying out the procedure.
Egg retrieval process
- A needle is inserted through the passed through the upper region of the vagina with the assistance of ultrasound for reaching up to the ovary and the growing follicles.
- The fluid of the follicles is aspirated with the help of the needle and the eggs are detached from the walls of the follicles for sucking out of the ovary.
- The mature cumulus oocyte complex is drawn from the follicle walls while aspirating the fluid using the needle.
- The entire process generally takes about ten minutes of time.
The eggs along with the fluid are then given to the IVF Laboratory for identifying the eggs, washing in culture medium and placing them in small drops in the culture dishes.
Recovery after the egg retrieval
- After aspirating all the follicles and the woman coming out of the sedation, she is closely monitored for nearly one hour or so and discharged to be taken back home.
- The woman may experience mild or moderate cramping after the procedure for some hours.
- Some of the women are given oral medicines, like Vicodin, for relieving their pain while discharging them from the clinic on the egg retrieval day.
How many eggs do we need for IVF?
- Normally, in one egg retrieval session, between 8 and 12 eggs are retrieved on an average.
- Your age plays a vital role on the count of egg retrieval process. The younger the woman, more the retrieved eggs.
What after the egg retrieval?
- After the egg retrieval process, eggs and sperm separated from the semen in the semen are placed together in the laboratory for nearly 4 hours. Or, the sperm are directly introduced into the eggs individually by injections. The procedure is known as ICSI.
- The next morning, the eggs are inspected for verifying their fertilization.
- The embryos, thus created, are cultured for 2 to 5 days in the IVF laboratory.
- Afterwards, one or two of the quality embryos are placed into your uterus using embryo transfer process and the rest are freezed using cryopreservation.
- 14 days after carrying out the egg retrieval process, we will be carrying out one of the pregnancy tests to know the good news for you.
- We also carry out the hCG blood test to confirm your pregnancy.